Become a part of our research team in Berlin to study RNA biology in neurons and its role in neurodegeneration. Our primary focus is Amyotrophic Lateral Sclerosis (ALS), a debilitating condition that impairs motor neurons, which are essential for skeletal muscle activity and motion. We aim to investigate how anomalies in RNA metabolism contribute to the progression of neural degeneration, thereby offering a deeper understanding of the disease's pathology.
What You'll Do:
• Use state-of-the-art methods, including systems biology techniques (e.g., Ribo-seq, SLAM-seq), biochemical assays, and various imaging approaches
• Employ human-induced pluripotent stem cells (hiPSCs)-based models of neurodegenration and CRISPR gene editing technologies
• Apply compartmentalized neuronal cultures, extending the existing body of work from our lab (Loedige et al. Molecular Cell 2023; Mendonsa et al. Nature Neuroscience 2023; Mendonsa et al. NAR 2021; Ludwik et al. Methods 2019; Zappulo et al. Nature Comm 2017).
• For more details on our research, please visit https://www.chekulaevalab.org
Who We're Seeking:
• An enthusiastic researcher with experience in molecular biology techniques and cell culture
• Previous expertise in RNA biology, neurodegeneration and hiPSC culture will be an advantage
How to Apply:
Compile your cover letter and CV, including contact information of at least two referees, into a singular PDF file. Direct your application package to marina.chekulaeva(at)mdc-berlin.de. We're looking forward to your application!
(1) Loedige I., Baranovskii A., Mendonsa S., Dantsuji S., Popitsch N., Breimann L., Zerna N., Cherepanov V., Milek M., Ameres S. and Chekulaeva M. mRNA stability and m6A are major determinants of subcellular mRNA localization in neurons. Molecular Cell 3;83(15):2709-2725.e10. https://doi.org/10.1016/j.molcel.2023.06.021
(2) Mendonsa S., von Kügelgen N., Dantsuji S., Ron M., Breimann L., Baranovskii A., Lödige I., Kirchner M., Fischer M., Zerna N., Bujanic L., Mertins P., Ulitsky I., and Chekulaeva M. (2023) Massively parallel identification of mRNA localization elements in primary cortical neurons. Nature Neuroscience 26: 394–405. https://www.nature.com/articles/s41593-022-01243-x
(3) Mendonsa S., von Kuegelgen N., Bujanic L. and Chekulaeva M. (2021). Charcot-Marie-Tooth mutation in glycyl-tRNA synthetase stalls ribosomes in a pre-accommodation state and activates integrated stress response. Nucleic Acids Research 49(17): 10007-10017. https://doi.org/10.1093/nar/gkab730
(4) Zappulo, A., van den Bruck, D., Ciolli Mattioli, C., Franke, V., Imami, K., McShane, E., Moreno-Estelles, M., Calviello, L., Filiipchyk, A., Peguero-Sanchez, E., Mueller, T., Woehler, A., Birchmeier, C., Merino, E., Rajewsky, N., Ohler. U., Mazzoni, E., Selbach, M., Akalin, A., and Chekulaeva, M. (2017). RNA localization is a key determinant of neurite-enriched proteome. Nature Communications 8(1): 583. https://dx.doi.org/10.1038/s41467-017-00690-6