Postdoctoral Training Fellow - Defining immunotherapy target regulation through CRISPR screens

The Translational Oncogenomics Lab (Lab head: Dr Marco Gerlinger) is looking for a highly motivated post-doctoral fellow to define the regulatory network that controls the expression of the immunotherapy target-antigen CEA in colorectal cancer organoids. The project will use genome-wide CRISPR screens and analyse the data with gene set enrichment analysis to identify candidate network components and genes that influence CEA expression. Key regulators will be validated with genetic perturbations and available drugs or tool compounds. This should inform the development of combination therapies of CEA-targeting bispecific antibodies and drugs that prevent antigen loss from cancer cells (Gonalez-Exposito et al. JITC 2019) and preclinical testing in immunocompetent in vitro models. The project funding is conditional on achieving milestones and the initial appointment is for 12 months with planned extension to 3 years.

Applicants with a PhD and up to 1 year of prior post-doctoral experience can apply. Experience in cell culture and molecular biology (molecular cloning and lentivirus work) is essential and previous use of CRISPR, siRNA and organoid technology would be beneficial. We expect prior publications and excellent writing, presentation and team working skills as well as a willingness to acquire skills in large scale data analysis (eg. with R studio) and in vitro T cell assays. Training in large scale CRISPR screening and data analysis will be provided through collaboration with Prof Lord, ICR.

The Translational Oncogenomics team consists of biologists, immunologists, bioinformaticians and clinicians and is highly collaborative. We are looking for a team player with a strong focus on delivering results and enthusiasm for translational cancer research.

Appointment will be on a Fixed Term Contract for 12 months in the first instance, with a starting salary in the range of £32,200 - £38,600 p.a. inclusive, depending on postdoctoral experience (£32,200 will be a starting salary for pre-viva new starters). The post will be based in Chelsea, London.

To apply, please submit online application on the ICR Careers site, attaching your CV with the supporting statement (addressing with specific examples where you meet the person specification, a brief description of your research experience and incl. the names and addresses of at least two referees).
Job reference: 1067